Fig. 3

Verification of PA-Flp-dependent Cre (PA-FdCre) system in ROSA26^RCE:FRT/Ai14 mouse line. a Schematic depicting AAVs carrying PA-Flp and Leak Free Flp-dependent Cre driver (LF-FdCd) as PA-Flp dependent Cre (PA-FdCre) systems. b Schematic depicting the generation of ROSA26^RCE:FRT/Ai14 mice. Flp reporter (GFP) and Cre reporter (TdTomato) alleles are located on the ROSA26 cassette in ROSA26^RCE:FRT/Ai14 mice. c, d Light-induced dual Flp and Cre activation in the hippocampal DG (c) or MS (d) of ROSA26^RCE:FRT/Ai14 mice (8–12-wk-old). Scale bar: 100 μm. e, f Correlation between GFP and TdTomato fluorescence intensity in the hippocampal DG (e) or MS (f) among PA-Flp–positive cells (magenta) in with (w/) LED and without (w/o) LED groups. A total of 200–500 cells were counted from each w/ LED and w/o LED group (n = 4 mice/group)