Fig. 1

TAZ stimulates Irs1 expression and insulin signalling. a Insulin was intraperitoneally injected into wild-type (WT) and muscle-specific TAZ-knockout (mKO) mice. After 15 min, muscle lysates were analysed by immunoblotting. Eight- to ten-week-old mice were used for experiment. b The three independent experiments shown in a were assessed, and the ratio of phosphorylated to total AKT was analysed, as well as the protein levels of ribosomal protein S6 kinase and AS160. c Serum-starved WT and TAZ-knockout (KO) mouse embryonic fibroblasts (MEFs) were treated with 1 nM insulin. Cell lysates were analysed by immunoblotting at the indicated time points. β-Actin was used as a loading control. d Serum-starved control (Con) and TAZ-knockdown (Ti) C2C12 myoblasts were treated with 1 nM insulin. Cell lysates prepared at the indicated time points were analysed by immunoblot analysis. β-Actin was used as a loading control. e Irs1 transcription was analysed by quantitative reverse transcription (qRT)-PCR in skeletal muscle from WT and mKO mice (n = 7, left), WT and TAZ-KO MEFs (n = 3, middle), Con and Ti C2C12 cells (n = 3, right). For skeletal muscle data, 8–10-week-old mice were used. Data are presented as mean ± SD for panels b and e (middle and right), and mean ± SEM for panel e (left). Statistical analysis was performed using a Student’s t-test. *p < 0.05; ***p < 0.005