Fig. 5

TAZ stimulates Wnt signalling-induced Irs1 transcription. a Wild-type (WT) and TAZ-knockout (KO) mouse embryonic fibroblasts (MEF) were treated with Wnt3a-conditioned or control medium from L cells, for 24 h. Cell lysates were analysed by immunoblotting. b Irs1 expression of cells described in a was analysed by qRT-PCR (n = 3). c Control (Con) and TAZ-knockdown (Ti) C2C12 myotubes were treated with control or Wnt3a-conditioned medium for 24 h. Level of IRS1 and TAZ was then analysed by immunoblotting. d Total RNAs from cells described in c were prepared, and Irs1 expression was analysed by qRT-PCR (n = 3). e Gastrocnemius muscles from WT, muscle-specific TAZ-knockout (TAZmKO), muscle-specific adenomatous polyposis coli (APC)-KO (APCmKO), and muscle-specific APC and TAZ double-KO (DbmKO) mice were analysed by immunoblotting, using the indicated antibodies. For β-catenin data, non-phospho (active) β-catenin antibody was used. f Irs1 transcription was analysed in the gastrocnemius muscles of WT, TAZmKO, APCmKO, and DbmKO mice by qRT-PCR (n = 8). g Glucose tolerance and h insulin tolerance were assessed in WT, TAZ-mKO, APC-mKO, and DbmKO mice (n = 6). For e–h, 8–10-week-old mice were used. Data are presented as mean ± SD for panels b and d, and as mean ± SEM for panels f–h. Statistical analysis was performed using a Student’s t-test. *p < 0.05; ***p < 0.005