Fig. 1
Wrp-BirA localizes to and biotinylates proteins within nascent dendritic spines. a Representative images of cultured neurons expressing either BirA (Control), Membrane-Tag-BirA (MT-BirA), or Wrp(IFBAR)-BirA (Wrp-BirA). Co-transfected tandem dimer Tomato fluorescent protein (tdTomato67) fill is shown in magenta, streptavidin staining is shown in green, and the HA tag for each construct is shown in blue. Scale bars are 5 μm. b Graph depicting streptavidin enrichment in dendritic protrusions vs the dendrite for each BirA (0.555 ± 0.110 spine intensity: dendrite intensity, n=6 neurons), MT-BirA (0.622 ± 0.113 spine intensity: dendrite intensity, n=6 neurons), and Wrp-BirA (1.353 ± 0.300 spine intensity: dendrite intensity, n=6 neurons). F2,15=5.138, p=0.020. c Graph depicting streptavidin enrichment in dendritic protrusions vs the dendrite for BirA (0.465 ± 0.080 spine intensity: dendrite intensity, n=6 neurons), MT-BirA (0.732 ± 0.118 spine intensity: dendrite intensity, n=6 neurons), and Wrp-BirA (2.269 ± 0.530 spine intensity: dendrite intensity, n=5 neurons). F2,8.591=6.452, p=0.019. Error bars are standard error of the mean (SEM). *p<0.05; **p<0.01, one-way ANOVAs
