Fig. 2
Proteome of developing dendritic protrusions. a Wrp-BirA-dependent iBioID identified a network of known and unknown proteins enriched in dendritic filopodia early in spinogenesis. Node titles correspond to the gene name, size represents fold enrichment over the BirA negative control (range 2.45–23.73), shading represents p-value with light gray being a lower p-value and darker gray a higher p-value (range 9.06 × 10−5–0.05). Edges are marked according to type of interaction. Dashed lines are iBioID interactions where as solid lines are previously known interactions identified in the Genemania, Biogrid, or String databases. b Clustergram of synaptic proteins (pink) as identified through DAVID analysis or the G2C database of Cortex PSD Consensus proteins38. c Clustergram of adhesion proteins (orange) as identified through DAVID analysis. d Clustergram of receptor proteins (green) as identified through DAVID analysis. e Clustergram of cytoskeletal proteins (blue) as identified through DAVID analysis. f Venn diagram of Wrp-BirA proteome compared to three previously published proteomes of mature postsynapses. g Clustergram of proteins found exclusively in the Wrp-BirA network in comparison to mature postsynaptic proteomes (purple)
