Table 1 Michaelis–Menten kinetic parameters
Levodopa | pH 5.0 | pH 5.0 | pH 4.5 | pH 7.4 |
---|---|---|---|---|
TDCEFS | TDCEFM | PTDCEFM | DDC | |
[E] (nM) | 10 | 10 | 10 | 10 |
Km (mM) | 3 ± 0.4 | 7.2 ± 0.8 | 0.4 ± 0.1 | 0.1 ± 0.01 |
Vmax (µM/min) | 35.3 ± 1.4 | 25.5 ± 1.3 | 3.4 ± 0.2 | 1.4 ± 0.03 |
Kcat (min−1) | 3531 ± 137 | 2549 ± 133 | 342.4 ± 21 | 136.9 ± 3 |
Kcat/Km (min−1/mM−1) | 1160 | 352 | 764 | 1567 |
R2 | 0.978 | 0.99 | 0.621 | 0.962 |
Tyrosine | pH 5.0 | pH 5.0 | pH 4.5 | |
TDCEFS | TDCEFM | PTDCEFM | ||
[E] (nM) | 10 | 10 | 10 | |
Km (mM) | 0.6 ± 0.1 | 1.5 ± 0.3 | 0.2 ± 0.05 | |
Vmax (µM/min) | 69.6 ± 2.9 | 22 ± 2.5 | 4.4 ± 0.2 | |
Kcat (min−1) | 6963 ± 288 | 2204 ± 247 | 435.6 ± 19.2 | |
Kcat/Km (min−1/mM−1) | 12216 | 1493 | 2558 | |
R2 | 0.928 | 0.902 | 0.589 |