Fig. 7

Constitutive activation of STAT5 partially rescues Treg cell dysfunction. a, b Representative images of mice (a) and peripheral LNs and spleen (b) from 2-week-old Foxp3^YFP-Cre/Y Ogt^fl/Y and Foxp3^YFP-Cre/YOgt^fl/Y Rosa26^Stat5b-CA/wt mice. c Survival curve of Foxp3^YFP-Cre/Y Ogt^fl/Y and Foxp3^YFP-Cre/YOgt^fl/Y Rosa26^Stat5b-CA/wt mice (n = 6). d MFI of FOXP3 in FOXP3⁺CD25⁺ Treg cells in the LNs from 2-week-old Foxp3^YFP-Cre/Y Ogt^wt/Y, Foxp3^YFP-Cre/Y Ogt^fl/Y and Foxp3^YFP-Cre/YOgt^fl/Y Rosa26^Stat5b-CA/wt mice, at least n = 3 each group. e Frequencies of FOXP3⁺CD25⁺ Treg cells among CD4⁺ T cells, at least n = 9 each group. f–g Frequencies of CD44^hiCD62L^lo (f) and CD44⁺KLRG1⁺ (g) eTreg cells among FOXP3⁺CD25⁺ Treg cells in the LNs from 2-week-old Foxp3^YFP-Cre/Y Ogt^wt/Y, Foxp3^YFP-Cre/Y Ogt^fl/Y and Foxp3^YFP-Cre/YOgt^fl/Y Rosa26^Stat5b-CA/wt mice, at least n = 6 each group. h Histogram of FOXP3 expression in CD4⁺CD25⁺YFP⁺ Treg cells in the LNs from 2-week-old Foxp3^YFP-Cre/Y Ogt^wt/Y, Foxp3^YFP-Cre/Y Ogt^fl/Y and Foxp3^YFP-Cre/YOgt^fl/Y Rosa26^Stat5b-CA/wt mice. i, j Absolute number of CD4⁺ (i) and CD8⁺ (j) T cells in the LNs, at least n = 3 each group. k, l Frequencies of CD44^hiCD62L^lo effector T cells in CD4⁺ (k) and CD8⁺ cells (l), at least n = 7 each group. m–o Frequencies of T-BET⁺ (m), GATA3⁺ (n) and RORγT⁺ (o) populations among CD4⁺FOXP3⁻ cells in the LNs, at least n = 6 each group. p Frequencies of INFγ, IL-4 and IL-17A-producing CD4⁺FOXP3⁻ cells from Foxp3^YFP-Cre/Y Ogt^fl/Y and Foxp3^YFP-Cre/YOgt^fl/Y Rosa26^Stat5b-CA/wt mice, at least n = 3 each group. Data are shown as mean ± s.e.m. *p < 0.05; **p < 0.01; ***p < 0.001 by Kaplan-Meier Analysis (c), unpaired student’s t-test (p) and one-way ANOVA (d–g, i–o)