Fig. 1

Metabolic regulation of T-helper type 1 (Th1) switching in primary human CD4⁺ T cells. a Schematic flow cytometric dot-plot of the life cycle of human Th1 switching; double-positive cells are highlighted with a blue gate, while IL-10-negative populations are shown within a black gate; b Heatmap of 111 significantly expressed genes in the double-negative (IFNγ⁻IL-10⁻) and single-positive (IFNγ⁺IL-10⁻) populations associated with changes in IL-10 protein expression, as measured by median fluorescence intensity ratio (MFI⁺:MFI⁻) for IL-10 expression in the double-positive (IFNγ⁺IL-10⁺) population. c Ingenuity Pathway Analysis (IPA) based on genes from b (SP: superpathway). Bars represent the percentage of the number of genes mapping to a particular pathway, coloured in green to note the inverse correlation. The number indicates the number of total genes ascribed to the pathway. The yellow line represents the P value as calculated by Fisher’s test and corrected for multiple testing using the Benjamini–Hochberg correction. d IPA based on genes differentially expressed between CYT-1- and CYT-2-expressing Jurkat T cells and analysed and annotated as in c