Fig. 5
From: Sensory neuron lineage mapping and manipulation in the Drosophila olfactory system
The at1 lineage is synchronized in cell division sequence and marker expression. a Full views of the immortalized at1 driver-labeled lineages (using an rCD2:GFP reporter; green) on pupal antennae at three developmental time points. For each time point, all the at1 lineages have comparable numbers of daughter cells and identical Svp (red) and Sens (blue) expression patterns (shown in the magnified views of the boxed regions). The histograms show the percentage of at1 lineages at different cell stages. Scale bar = 20 µm in this and other panels. b Close-up views of the SOP and its daughter cells in the at1 lineage (green) reveal a highly stereotyped series of cell divisions from 2 to 8 cells, before the Nbb, Naa and Nba cells undergo sequential apoptosis. Most of the intermediate and terminal daughter cells in this lineage could be unambiguously identified based on the expression of Svp (red) and Sens (blue), as well as their position within the cluster (division typically occurs along the same axis). For the last apoptotic Nba stage, Elav (red) marks the neural-specific Nab and Nba cells. Where necessary, multiple focal planes are shown to visualize all the cells in each cluster. The yellow or magenta dashed lines within each cell stage outline the same at1 cluster at different focal planes (across multiple panels). c Schematic showing the division time, birth sequence and expression pattern of molecular markers in the at1 lineage (based also upon data from Supplementary Fig. 3c–e)
