Fig. 6

An RNAi screen for molecular determinants of at1 lineage development. a RNAi screen workflow: two rounds of screening, starting from 808 candidate genes (see Methods) identified 35 genes that showed reproducible defects in antennal development. b Temporal expression of GAL4 driver lines used for RNAi: the constitutive driver, comprising ey-FLP and an actin-GAL4 flip-out cassette, is expressed specifically in the eye-antennal tissue from the second instar larval stage onwards, spanning SOP specification, divisions and OSN differentiation (red bar). The late peb-GAL4 driver is expressed only after OSNs begin to differentiate (green bar). c Examples of the six phenotypic categories. Left: lateral view of the fly head, with the eyes and antennae marked by arrows. When Eip93F RNAi is induced with the constitutive driver, both antennae and eyes display morphological defects, in addition to loss of eye pigmentation. Right: right antennal lobes of control and the indicated RNAi animals expressing an Or67d-CD8a:GFP reporter (green), which labels the axons of the Or67d and Or82a OSNs that innervate the DA1 and VA6 glomeruli (yellow arrows), respectively. RNAi phenotypes of specific genes with the constitutive driver include global glomerular morphology defects, axon density alteration, imprecise axon targeting, precise targeting errors and/or other defects (indicated by the white arrows). Scale bar = 20 µm. d Phenotypic classification of the 35 high confidence screen hits when the RNAi transgenes were driven by either the constitutive or late driver. Each gene is classified under six phenotypic categories in three levels of phenotypic severity