Fig. 2
From: SMARCA4 loss is synthetic lethal with CDK4/6 inhibition in non-small cell lung cancer
Palbociclib is effective against SMARCA4-deficient non-small cell lung cancer (NSCLC) tumor growth in vivo. Palbociclib inhibits tumor growth in xenograft models of H1299 (a, b, e, f) and H1703 (c, d, g, h). a, c Tumor size from day 0 of treatment in H1299 (a, n = 4 per group) and H1703 (c, n = 8 for vehicle, n = 7 for palbociclib; 150 mg kg−1) models. Error bars represent mean ± standard error of mean (s.e.m.); two-way analysis of variance (ANOVA), ****p < 0.0001. b, d Final tumor weight measured after surgery in H1299 (b) and H1703 (d) models. Two-tailed t-test, **p < 0.01, ****p < 0.0001. e–h Palbociclib treatment resulted in suppression of RB phosphorylation, Ki67 expression and mitotic index in xenograft tumors of the trial endpoints. Representative images of Immunohistochemistry (IHC) (p-RB, Ki67) and hematoxylin and eosin (H&E) analysis of H1299 (e) and H1703 (g) xenograft tumor tissues. Bar 50 µm; black arrows point to mitotic active cells as examples. f, h Quantifications of p-RB, Ki67 and mitotic count of H1299 (f, n = 3) and H1703 (h, n = 4). Two-tailed t-test, **p < 0.01, ***p < 0.001, ****p < 0.0001
