Fig. 1
From: Remodeling of secretory lysosomes during education tunes functional potential in NK cells
NK cell education is associated with accumulation of granzyme B. a CD107a expression in response to K562 cells by the indicated KIR subset of resting mature CD56dim NKG2A− NKG2C−CD57− NK cells from C1/C1 (n = 38 donors), C1/C2 (n = 31 donors), and C2/C2 (n = 19 donors). b Expression of granzyme B in 2DL3 and 2DL1 single-positive NKG2A−CD57− NK cells from C1/C1 (n = 21 donors), C1/C2 (n = 26 donors), and C2/C2 (n = 12 donors). Donors with less than 100 events in the final KIR gate were excluded. c Expression of granzyme B in 3DL1+/− NKG2A−CD57− NK cells from Bw4+ (n = 20 donors) and Bw4− (n = 7 donors). d Expression of granzyme B in 2DL2/2DS2 or 2DL3-single-positive NKG2A− NKG2C−CD57− NK cells in C1/C1 (n = 9 donors), C1/C2 (n = 8 donors), and C2/C2 (n = 9 donors). e Expression of granzyme B in the indicated NKG2A− NKG2C−CD57− NK cell subset in C2/C2 (n = 7 donors). f Expression of granzyme B in KIR−CD57−NKG2A+/− CD57− NK cells (n = 63 donors). g Expression of granzyme B in YTS and NKL cells transfected with 2DL3 or 2DL1. The graph shows data from one representative experiment of two. Paired t-tests were performed in (a–c, f–g). One-way ANOVA tests followed by Tukey's multiple comparison tests were performed in (e, f). Whiskers show 5th to 95th percentile. Bars show the median. ****p < 0.0001; ***p < 0.001; and *p < 0.05. Red and blue circles and box plots represent NK cells with self or non-self KIR, respectively
