Fig. 2

Granzyme B accumulation is independent of transcription. a Global RNA-Seq of sorted self-KIR⁺ and non-self-KIR⁺ NKG2A⁻ NKG2C⁻CD57⁻ NK cells. The figure depicts one representative C1/C1 donor out of three independent donors. b Quantitative PCR of granzyme B mRNA in sorted NK cells at different stages of differentiation (left, 4 pooled samples) and in NKG2A⁻NKG2C⁻CD57⁻ NK cells expressing a self- or non-self KIR (right, n = 5 paired samples). c Expression of granzyme B in the indicated NK cell subsets following stimulation with IL-15 or IL-21 for the indicated length of time (summary of two independent experiments using two different donors). d Expression of granzyme B after 24 h of stimulation with IL-21 or IL-15 in CD56^bright NK cells and NKG2A⁻ NKG2C⁻CD57⁻ single-positive CD56^dim NK cells expressing a self- or non-self KIR NK cell subset in the presence or absence of the STAT-5 inhibitor Pimozide (top) (7 μM) and the mTOR inhibitor Torin-1 (100 nM) (bottom). The data in panel (d) are the summary of at least two independent experiments. Whiskers show 5th to 95th percentile. Bars show the median. Wilcoxon paired, non-parametric tests. ****p < 0.0001 and **p < 0.01. Red and blue circles, connecting lines and box plots represent NK cells with self or non-self KIR, respectively