Fig. 5

Compromising lysosomal activity decreases NK cell function. a Global Ca²⁺-flux in resting bulk NK cells measured by Fluo-4 F₁/F₀ ratio following stimulation with biotinylated anti-DNAM-1/anti-2B4 (top) or biotinylated anti-CD16 (bottom) crosslinked at the indicated time-point with streptavidin in the presence (added at the onset of stimulation and maintained throughout the assay) or absence of 50 μM GPN. b Representative example of CD107a expression following stimulation of NK cells with K562 cells in the presence or absence of GPN. c Frequency of CD107^high+ and d IFNγ⁺ self-KIR⁺ and non-self-KIR⁺ NK cells following stimulation with K562 cells in the presence or absence of 50 μM GPN or 10 μM Mefloquine (MEF). Friedman’s tests were performed followed by Dunn’s multicomparison tests. Whiskers show 5th to 95th percentile. Bars show the median. ****p < 0.0001; ***p < 0.001; **p < 0.01; and *p < 0.05. Red and blue box plots represent NK cells with self or non-self KIR, respectively