Fig. 7
From: Functional role of PGAM5 multimeric assemblies and their polymerization into filaments
Multimerization of full-length PGAM5 promotes clustered mitochondrial morphology. a Representative confocal images of COS7 cells transiently transfected with the wild type (WT) or mutant full-length myc-tagged PGAM5 constructs and immunostained for the OMM marker Tom20 with anti-Tom20 antibody (green), PGAM5 with anti-myc antibody (red), and nucleic-stain DAPI (blue). All scale bars correspond to 15 µm. b Quantification of mitochondrial phenotypes observed by confocal microscopy in COS7 cells transiently expressing PGAM5 variants as described in a, based on at least three independent experiments per construct. Data are represented as mean ± S.E.M., determined using GraphPad Prism. Untransfected cells: n = 122 cells over three experimental replicates. Cells expressing PGAM5 WT: n = 296 cells over seven experimental replicates. Cells expressing PGAM5 H105A: n = 141 cells over three experimental replicates. Cells expressing PGAM5 R288E: n = 212 cells over five experimental replicates. Cells expressing PGAM5 A4: n = 144 cells over three experimental replicates. Cells expressing PGAM5 F244E: n = 186 cells over four experimental replicates. Cells expressing PGAM5 Y198E: n = 108 cells over three experimental replicates. Cells expressing PGAM5 R65A: n = 141 cells over four experimental replicates
