Fig. 6 | Nature Communications

Fig. 6

From: Corticosteroids inhibit Mycobacterium tuberculosis-induced necrotic host cell death by abrogating mitochondrial membrane permeability transition

Fig. 6

p38 MAPK inhibition preserves intracellular ATP levels. a, b Intracellular ATP levels in Mtb-infected J774.2 Mφ (a) and MRC-5 lung fibroblasts (b) following 24 and 48 h of infection, respectively. Cells were treated with dexamethasone (5 µM) or doramapimod (10 µM). c Intracellular ATP levels in Mtb-infected J774.2 Mφ treated with dexamethasone (5 µM) and bromopyruvic acid (3BP) (5 µM) or doramapimod (10 µM) and bromopyruvic acid (5 µM) after 24 h of infection. Data from two experiments with multiple replicates are shown in a and b and data from one of two experiments are shown in c. Results are expressed as mean ± SEM and experiments were analyzed using unpaired t-test (**p ≤ 0.01; ***p ≤ 0.001)

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