Fig. 3
From: Modular Design of Programmable Mechanofluorescent DNA Hydrogels

Strain and temporal response of the mechanofluorescent DNA hydrogels with different force-sensing modules. a Sequence and structure of the different modules investigated. Note that the rest of the modules, fluorophores (Atto488, Atto565), quencher (Iowa Black RQ), and attachment strands are identical in all experiments. The T and N modules are controls in which the fluorophore and quencher strands are (T) connected via a T6 covalent link without sacrificial duplex or not connected (N) at all. b Fluorescence imaging at different elongations of a 0.7 wt% hydrogel functionalized with D1. Scale bar = 1 mm. c Evolution of the red/green fluorescence ratio R/G(ε) after subtraction of R/Gini for the different DNA hydrogels functionalized with the mechanofluorescent modules. d Temporal recovery of the fluorescence ratio after hydrogel failure (normalized to R/Gmax and R/Gini)