Table 1 Overview of the DNA sequences and their functions and domains
From: Modular Design of Programmable Mechanofluorescent DNA Hydrogels
Function | Name | Sequence 5′→3′ |
|---|---|---|
Hydrogel matrix | Hydrogel precursor A (A1A2XA3A4) | (GGTGGCGGCTGACTG GTCAATGAATCGCGT CCGACGTTGACG CTGGATGTAGGATGC GGCGTGTCCACCTAC)~300 |
Hydrogel precursor B (B1B2X*B3B4) | (GCGAAGCGCCCGCTG CCTGTTGAGCGTATC CGTCAACGTCGG GCTGTACCGTTATTG CTCGCGCGGCAGCTC) ~300 | |
Common fluorescent strands | Fluorescent arm Atto565-MA4* | Atto565-CGCGTTGCGCCTGCC GTAGGTGGACACGCC |
Quencher arm B1*N-IowaRQ | CAGCGGGCGCTTCGC GAGCCGCGCACGCCG-IowaRQ | |
Reference Atto488-A2* | Atto488-ACGCGATTCATTGAC | |
Sensor D1 | Arm D1* (A1*M*D1*) | CAGTCAGCCGCCACC GGCAGGCGCAACGCG CGGCCGCGCGCCCGG |
Arm D1 (D1N*B4*) | CCGGGCGCGCGGCCG CGGCGTGCGCGGCTC GAGCTGCCGCGCGAG | |
Sensor D2 | Arm D2* (A1*M*D2*) | CAGTCAGCCGCCACC GGCAGGCGCAACGCG CGTCCGAC |
Arm D2 (D2N*B4*) | GTCGGACG CGGCGTGCGCGGCTC GAGCTGCCGCGCGAG | |
Sensor HP | Arm HP (A1*M*HPN*B4*) | CAGTCAGCCGCCACC GGCAGGCGCAACGCG CGTCCGACTTTTTTGTCGGACG CGGCGTGCGCGGCTC GAGCTGCCGCGCGAG |
Control T | Arm T (A1*M*TN*BB4*) | CAGTCAGCCGCCACC GGCAGGCGCAACGCG TTTTTT CGGCGTGCGCGGCTC GAGCTGCCGCGCGAG |
