Fig. 1 | Nature Communications

Fig. 1

From: PLCβ2 negatively regulates the inflammatory response to virus infection by inhibiting phosphoinositide-mediated activation of TAK1

Fig. 1

RNA virus infection specifically induces PLCβ2 expression. a Protein expression ratio of white blood cells mixture from control and HFMD group patients detected by mass spectrometry. b Immunoblot (IB) and analysis of PLCβ2 in PBMC from control and HFMD patients. c, d IB of PLCβ2 in lysates of mouse peritoneal macrophages infected with CVA16 virus (c) or stimulated with poly(I:C) (d) for the indicated times. e IB of PLCβ2 in wild-type or Tlr3−/− mouse peritoneal macrophages stimulated with poly(I:C) for the indicated times. f IB of PLCβ2 in mouse peritoneal macrophages pretreated for 1 h with the p38 inhibitor SB203580 before poly(I:C) stimulation for the indicated times. *p < 0.05 by unpaired t-test (b). Data are representative of three experiments with at least three independent biological replicates (mean and s.e.m. of n = 6 per group in b and n = 3 cultures in c–f)

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