Fig. 3 | Nature Communications

Fig. 3

From: PLCβ2 negatively regulates the inflammatory response to virus infection by inhibiting phosphoinositide-mediated activation of TAK1

Fig. 3

PLCβ2 interacts with TAK1. a–c IB or immunoprecipitates (IP) of lysates from HEK293T cells transfected with various plasmids as indicated. d In vitro GST precipitation assay of TAK1 and PLCβ2. e Precipitation of endogenous PLCβ2 from RAW264.7 cells expressing GST or GST-TAK1. f Endogenous interaction of PLCβ2 with TAK1 in poly(I:C)-treated primary macrophages. g Confocal analysis of colocolization of TAK1 and PLCβ2 in peritoneal macrophages stimulated with poly(I:C) for 4 h. Scale bar, 5 μm. h IB or IP of lysates from HEK293T cells transfected with FLAG-tagged PLCβ1-3 and HA-tagged TAK1. Data are representative of three experiments with at least three independent biological replicates (n = 3 cultures in f, g)

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