Fig. 7 | Nature Communications

Fig. 7

From: PLCβ2 negatively regulates the inflammatory response to virus infection by inhibiting phosphoinositide-mediated activation of TAK1

Fig. 7

PLCβ2 activator alleviates the pathogenesis of CVA16 infection. a, b Immunoblot analysis of lysates from macrophages pretreated with DMSO or m-3M3FBS, and then stimulated with poly(I:C) for the indicated times using different phospho-antibody. c–e Q-PCR analysis of relative Tnf (c), Il6 (d) and Il12p40 (e) mRNA in macrophages pretreated with DMSO or m-3M3FBS, then stimulated with poly(I:C) for the indicated times. Fourteen-day-old wild-type mice were pretreated with DMSO or m-3M3FBS and subsequently infected with CVA16 for 0 or 5 days. f–i Survival rate (f), clinical score (g) and histopathology in lung (h) or skeletal muscle (i) tissue of mice treated as above at either day 0 (not infected) or day 5. Scale bar, 50 μm. j Diagram. *p < 0.05; **p < 0.01 and ***p < 0.001 by unpaired t-test (c–e). Gehan–Breslow–Wilcoxon test (f) and two-way analysis of variance plus Bonferroni’s posttest (g) were used to calculate p value. Data are representative of three experiments with at least three independent biological replicates (mean and s.e.m. of n = 3 cultures in a–e or n = 6 mice per group in f–h)

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