Fig. 1
From: BMP-dependent synaptic development requires Abi-Abl-Rac signaling of BMP receptor macropinocytosis
Characterization of Drosophila abi gene, mutants, and neuromuscular junction (NMJ) expression. a Genomic organization of the Drosophila abi locus showing exon/intron organization of abi and two neighboring genes (twf and 140up), position of P-elements (G6718 and G4355), and abi5 deletion generated by G6718 excision. Untranslated regions, white boxes; translated regions, black boxes; translation start sites, arrows. Gray bar represents the abi-GAL4 promoter region. b Reverse transcription-PCR analysis of abi, twf, and rp49 RNA expression in wild type (WT; w1118), abi5, and abi5/Df. c Quantification of adult viability for wild type, abi5/Df, abi-GAL4/+; UAS-HA-abi,Df/abi5 (abi-GAL4 rescue), da-GAL4,abi5/UAS-HA-abi,Df (da-GAL4 rescue), C155-GAL4/+; UAS-HA-abi,Df/abi5 (C155-GAL4 rescue), 24B-GAL4,abi5/UAS-HA-abi,Df (24B-GAL4 rescue), and C155-GAL4/+; 24B-GAL4,abi5/UAS-HA-abi,Df (C155, 24B-GAL4 rescue) animals. The number of abi5/Df flies is given as a percentage of the expected viability, which is half the number of adults carrying a balancer chromosome. Values are from three independent experiments and presented as percentages of wild type. d Quantification of response time in the larval roll-over assay for the indicated genotypes. e Western blot of third instar larval extracts probed with anti-Abi and anti-β-actin. Numbers are molecular masses in kDa. f Abi is enriched at NMJ boutons. Single confocal slices of NMJ 6/7 in wild type and abi5/Df co-labeled for anti-Abi and anti-HRP (top) or anti-Dlg (bottom). Scale bars: 2 μm. Bar graphs show mean ± s.e.m. The number of animals analyzed in at least three experiments is indicated above (c) or inside (d) bars. Statistical analyses were performed by one-way analysis of variance with Tukey–Kramer post hoc test. Comparisons are with wild type (*P < 0.001; **P < 0.01)
