Fig. 8
From: BMP-dependent synaptic development requires Abi-Abl-Rac signaling of BMP receptor macropinocytosis
Rabankyrin and C-terminal-binding protein (CtBP) inhibit bone morphogenetic protein (BMP)-dependent synaptic development. a Presynaptic Rabankyrin or CtBP RNA interference (RNAi) induces BMP-dependent synaptic overgrowth. Sample images of anti-HRP-labeled neuromuscular junctions (NMJs) 6/7 and 4 in the indicated genotypes. Insets show high-magnification boutons marked by asterisks. Satellite boutons are indicated by arrowheads. b Quantification of total (top) and satellite (bottom) bouton number at NMJ 6/7. Note that synaptic overgrowth with Rabankyrin or CtBP knockdown is sensitive to wit level. c Presynaptic Rabankyrin or CtBP RNAi increases P-Mad in motor neurons. Single confocal sections of NMJ 6/7 (top) and ventral nerve cord (VNC; bottom) co-labeled for anti-P-Mad and anti-HRP (NMJ) or anti-Eve (VNC). d Quantification of P-Mad intensity normalized to HRP or Eve. Bar graphs indicate mean ± s.e.m. The number of NMJs (b), NMJ branches (d, upper panel), or Eve-positive motor neurons (d, lower panel) analyzed for each genotype is indicated inside bars. All comparisons against C155-GAL4/+ unless indicated (one-way analysis of variance with Tukey–Kramer post hoc test; *P < 0.001; **P < 0.01; n.s. not significant). Scale bars: a, 20 μm; c, 2 μm (NMJ); c, 5 μm (VNC)
