Table 2 Quantitative cross-linking (qCL) of mTXNPxred and mTXNPxox using DSS
From: Chaperone activation and client binding of a 2-cysteine peroxiredoxin
Light-CL | Heavy-CL | CL-Peptides | Log2 (H/L)-average | Favored CL |
---|---|---|---|---|
Red (30 °C) | Red (30 °C) | 1–1 | −0.04 | – |
Red (30 °C) | Red (30 °C) | 1–1 | −0.02 | – |
Red (30 °C) | Ox (30 °C) | 1–3 | −2.09 | Red (30 °C) |
Red (30 °C) | Ox (30 °C) | 1–1 | −1.71 | Red (30 °C) |
Red (42 °C) | Red (30 °C) | 1–2 | 1.40 | Red (30 °C) |
Red (30 °C) | Red (42 °C) | 1–3 | −2.57 | Red (30 °C) |
Red (30 °C) | Red (42 °C) | 1–1 | −2.32 | Red (30 °C) |
Red (30 °C) | Red (42 °C) | 1–3 | −2.21 | Red (30 °C) |
Red (30 °C) | Red (42 °C) | 1–2 | −1.55 | Red (30 °C) |