Fig. 7
PIK3R1 loss renders cells sensitive to JAK2, STAT3, and AKT inhibitors and co-targeting JAK2/STAT3 and AKT induces synergism. a SKOV3 cells transfected with PIK3R1 siRNA were allowed to form 3D spheroids for 7 days prior to treatment with indicated inhibitors for 72 h. Dose–response curves of each inhibitor are shown. b IC50 values of each inhibitor were calculated by nonlinear regression analysis and are shown as heatmap. c SKOV3 3D spheroids were treated with each inhibitor alone or in combination as indicated, dose–response curves are shown. d Combination Index (CI) values were calculated with Chou-Talalay Method and CI values at the highest doses of the inhibitors used are visualized as heatmap. Error bars represent SD. *p < 0.05; **p < 0.005; ***p < 0.001 compared with mock using t-test
