Fig. 4
From: Hepatic Sdf2l1 controls feeding-induced ER stress and regulates metabolism
Generation and phenotypes of the knock out model of Sdf2l1. Sdf2l1-floxed mice were administered with Ad-Cre (3.0 × 107 PFU/g BW), to generate a liver-specific Sdf2l1 knockout model in adults. a Detection of deleted exon 2 of the Sdf2l1 gene by PCR. b, d–i Metabolic phenotypes analyzed 3–6 weeks after the adenovirus administration (n = 8–9): b body weight, d ad libitum-fed plasma glucose, e plasma glucose in insulin tolerance test (ITT), after intraperitoneal injection of human regular insulin (0.75 U/kg BW), f plasma glucose levels in an pyruvate tolerance test, after intraperitoneal injection of pyruvate (1.5 g/kg BW), g plasma glucose levels in an oral glucose tolerance test (OGTT), after oral administration of glucose (0.75 g/kg BW), following 16 h of fasting. c ER stress in the liver (n = 3–6), analyzed by RT-PCR. h, i Effects on lipid metabolism, analyzed with h triglyceride contents quantification (n = 3–6), and i Oil Red O staining. Values of the data are expressed as mean ± SEM. *P < 0.05, **P < 0.01. Unpaired 2-tailed t-test was used for assessment. Scale bars: 100 μm
