Fig. 1 | Nature Communications

Fig. 1

From: Development of human cGAS-specific small-molecule inhibitors for repression of dsDNA-triggered interferon expression

Fig. 1

Establishment of a high-throughput screening assay for identification of small-molecule h-cGAS inhibitors. a Schematic showing dsDNA-activated cGAS synthesis of cyclic GMP–AMP (cGAMP) from ATP and GTP. b, c Assessment of enzymatic activity of h-cGAS under varying dsDNA concentrations based on RapidFire mass spectrometry (RF-MS) assay (b) or ATP-coupled luminescence (LUM)-based assay (c). d Schematic for high-throughput screening of small-molecule h-cGAS inhibitors using assay (c). Numbers in parentheses indicate the column(s) in plate into which the specified reagent was dispensed. RT room temperature. (n = 6 for b and c; mean ± S.D.)

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