Fig. 2 | Nature Communications

Fig. 2

From: Development of human cGAS-specific small-molecule inhibitors for repression of dsDNA-triggered interferon expression

Fig. 2

ATP-coupled luminescence-based high-throughput screening results for h-cGAS. a Sigma-Aldrich LOPAC (library of 1268 pharmacologically active compounds) were tested at 12.5 µM final concentration for h-cGAS LUM assay. Normalized percent of inhibition (NPI) for each compound tested (gray dots) in two independent days were plotted against each other. Blue dots indicate DMSO negative control and red dots indicate the positive control (no dsDNA). b Scatter plot for the NPI of the 281,348 library compounds tested at 12.5 µM final compound concentration. Sample ID indicates the unique number assigned for each compound in the library. Gray dots, library compound; blue dots, negative control; red dots, positive control. c Z-prime factor analysis of each library plate. Plate ID indicates the unique number assigned for each library compound plate

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