Fig. 2

Differentially expressed long non-coding RNAs (lncRNAs) in each B cell subset. Unsupervised principal components analysis (PCA) of RNA-sequencing (RNA-seq) data for all a lncRNAs and b coding genes. Phylogenetic tree analysis of RNA-seq data for all c lncRNAs and d coding genes. e New scheme of B cell differentiation during the humoral immune response in which the memory B cells are positioned between the NB and GC B cells. The numbers show the distance between two of the B cell subpopulations. f The number of differentially expressed lncRNAs and g the percentage of differentially expressed novel lncRNAs, annotated lncRNAs, and protein-coding genes (with respect to total of expressed novel lncRNAs, annotated lncRNAs, and protenin-coding genes during the humoral immune response) between NB and CB, CB and CC, CC and MEM, CC and TPC, and TPC and BMPC. NB: naive B cells; CB: centroblasts; CC: centrocytes; MEM: memory B cells; TPC: tonsillar plasma cells; BMPC: plasma cells from bone marrow of healthy donors