Fig. 8
Identification of circular RNAs (circRNAs) during the human humoral immune response. a Scheme of pipeline used to define and identify the putative circRNAs expressed during the humoral immune response. b Box plots showing the average expression of total junction reads from circRNAs. c Box plots showing the expression level of MKI67, d PCNA, e ADAR1, f DHX9, and g HNRNPL genes in different B cell subpopulations. Analysis of variance (ANOVA) test was used for statistical analysis. h, i Box plots showing the average expression of junction reads derived from immunoglobulin (Ig) genes and junction reads derived from non-Ig genes. j Schematic representation of the circRNA formation by back splicing circularization and primers designed to properly amplify by PCR these circRNAs. k, l Cloning and sequencing results of a circRNA derived from Ig locus in normal tonsillar plasma cells or plasma cells from multiple myeloma patients as indicated. Box plots show the median as center, first and third quartiles as the box hinges, whiskers extend to the smallest and largest value no further than the 1.5× interquartile range (IQR) away from the hinges. NB: naive B cells; CB: centroblasts; CC: centrocytes; MEM: memory B cells; TPC: tonsillar plasma cells; BMPC: plasma cells from bone marrow of healthy donors; Ig: immunoglobulin genes; MM: multiple myeloma
