Fig. 1
Biochemical characterisation of the ASPP/PP1 complex. a Summary of protein-protein interactions in the ASPP/PP1 complex detected by AP-MS from ref. 38 (solid blue lines) and collated from the literature (dashed red lines). The baits used in ref. 38 are shown as hexagons, preys are shown as circles. b–e Western blots of co-IP experiments using lysates of transfected S2 cells, probed with indicated antibodies. b Mutation of V812 and F814 to alanine within the RVxF motif of ASPP (ASPPFA) decreases the binding between PP1 isoforms and ASPP). c RASSF8 binds to PP1α96A via ASPP. RASSF8 and PP1α96A co-IP with each other only when ASPP is present. d Increased PP1α96A/Ccdc85 co-IP in the presence of ASPP. e RASSF8 and Ccdc85 co-IP with each other only when ASPP is present
