Fig. 6

Affinity measurement for PP1α:ASPP2 complex. a ITC measurement of ASPP2^920-1120:PP1α^7-330 and ASPP2^920-1120:PP1α^7-300. b BLI measurement of ASPP2^920-1020 binding to immobilized PP1α^7-330. c Affinity measurement of various human PP1 C-tail peptides by ITC. The PPII motif is highlighted in grey, glycine and proline residues in the C-terminal region are highlighted in red and C-terminal basic residues in blue. d Affinity measurement of various PP1:ASPP complexes by BLI. The following proteins were used for these assays: ASPP2^920-1120, ASPP1^882-1090, iASPP^621-828, PP1α^7-330, PP1β^6-327, PP1ɣ^7-323. e ITC measurement of various PP1α^301-330:ASPP complexes