Fig. 3

Molecular characteristics associated with RPV in HGSOC. Gene set enrichment analysis identified a RPV-positively correlated biological pathways and b RPV-negatively correlated biological pathways from KEGG pathway database (FDR < 0.05). NES normalized enrichment score. c Volcano plot showing the differential expressed genes between stroma and tumor epithelial component from a public dataset, GSE40595. The genes that positively correlated with RPV are highlighted in red (r > 0.3, Spearman correlation); genes that are negatively correlated with RPV are highlighted in blue (r < −0.3, Spearman correlation). d Heatmap showing correlation of protein expression (Fibronectin, Rad51 and FoxM1) with RPV for 47 cases in the TCGA validation dataset. The significance between these protein features with RPV was indicated for 119 cases in the HH cohort and with eRPV from 353 additional TCGA cases. Top panel, RPV ranked from low to high (left to right) and their corresponding eRPV (light blue). Lower panel, protein expression level of Fibronectin, Rad51 and FoxM1. p values are given by one-sided Spearman’s correlation test as validation of the transcriptomic analyses. e Clinical, histological and genetic characteristics associated with RPV in the TCGA and HH cohorts. Each rectangle block represents one patient in the TCGA validation dataset. The significance of association between these characteristics with RPV in the TCGA validation dataset, HH cohort and eRPV in additional TCGA dataset is indicated on the right side. The significance is indicated on the right from Kruskal−Wallis test (molecular subtype) or two-tailed Wilcoxon rank-sum test (others). The association between RPV and stromal component is shown in (a), (c), (d) and (e); The association between RPV and proliferation or DNA damage response is highlighted in (b) and (d). ***p < 0.001, **p < 0.01, *p < 0.05, ns p > 0.1. RPV radiomic prognostic vector, HGSOC high-grade serous ovarian cancer, TCGA the Cancer Genome Atlas, KEGG Kyoto Encyclopedia of Genes and Genomes