Fig. 1
From: Extreme slow growth as alternative strategy to survive deep starvation in bacteria
Long-term survival of non-sporulating B. subtilis. a Colony-forming units (CFU) of B. subtilis ΔspoIIE (strain DG001) incubated in Spizizen minimal medium (SMM). b CFU of ΔspoIIE cells that were first grown for 2 days in SMM, and subsequently filtered and incubated in either starvation buffer or water (=0 days time point). The CFU numbers of the first time point are therefore comparable to those of time point 2 days in graph (a). Averages and standard deviation from three independent experiments are depicted. The difference between the two graphs becomes significant after day 7 (p < 0.05, unpaired two-tailed t-test). c CFU of spores and cells in a wild-type B. subtilis culture (strain BSB1) incubated in starvation buffer. The percentage of spores is indicated in the bar diagram. Results of two replicate experiments are shown in Supplementary Figure 2A. See Methods for details on growth and starvation conditions
