Fig. 6
From: Extreme slow growth as alternative strategy to survive deep starvation in bacteria
Presence of cell division sites. B. subtilis ΔspoIIE cells encoding divIVA-gfp reporter were incubated for 14 days in starvation buffer. a At different time intervals samples were taken for microscopic analysis. Cells were stained with the fluorescent membrane dye FM5–95. Scale bar is 2 µm. b To avoid bias, the captured phase contrast images were used to select approximately 100 cells per sample, and the number of cells containing a midcell DivIVA-GFP signal, indicative of cell division, were counted. As a control, the number of division sites present in both exponentially growing cells (OD600 ~0.2), and stationary growth phase cells (overnight) were determined. Bar diagram represents average and standard deviation of three independent experiments. Strain used: B. subtilis DG001 (spoIIE::erm, divIVA:divIVA-GFP(cm))
