Fig. 2
From: Suppression of autophagic activity by Rubicon is a signature of aging
Rubicon knockdown ameliorates age-dependent phenotypes in worms. a Transgenic worms expressing polyQ35::YFP in body wall muscle exhibited age-dependent accumulation of polyQ inclusions (open arrowheads) at day5 stage. rub-1 knockdown decreased the accumulation of inclusions, and concomitant knockdown of bec-1 reverted the phenotype. Each RNAi is indicated. Knockdown was conducted from egg onward. b Number of polyQ inclusions per worm in a. More than 20 worms were analysed in each experiment, and the experiments were repeated three times. Values represent means ± s.e.m. (n = 3). P value (*P < 0.05, **P < 0.01) was determined by t-test. c Locomotion speed, calculated from multi-worm tracking analysis at day5. rub-1 knockdown worms maintain high locomotion activity. Knockdown was conducted from egg onward. Values represent means ± s.e.m. (control, n = 12; rub-1, n = 8; atg-18, n = 8; rub-1 + atg-18, n = 13). P value (*P < 0.05) was determined by t-test. The representative picture of the tracking is shown in supplementary fig. 6a. d rub-1 knockdown increased the oxidative stress resistance in an autophagy dependent manner. Survivorship of wild-type day 1 worms subjected to indicated RNAi after 4.4 mM H2O2 treatment for 2 h. Knockdown was conducted from egg onward. Values represent means ± s.e.m. (n = 3). P value (**P < 0.01) was determined by t-test. Scale bars 50 μm (a)
