Fig. 3
From: Suppression of autophagic activity by Rubicon is a signature of aging
Tissue specific roles of Rubicon contributing to lifespan regulation in worms. a, b Neuronal knockdown of rub-1 extended lifespan efficiently. The longevity by rub-1 knockdown was abolished by concomitant atg-18 knockdown. TU3401(neuron specific RNAi sensitive strain) was used and knockdown was conducted from egg onward. See Supplementary Data 1 for details and repeats. c Neuron specific knockdown of rub-1 significantly increased no. of GFP::LGG-1 2 h after BafA injection compared to DMSO injection in neuron, while control knockdown did not, indicating autophagy flux was increased by rub-1 knockdown in neuron. Newly generated strains by crossing between MAH215 and TU3401 were used and images were taken at day 1 adult stage. Knockdown was conducted from egg onward. Arrowheads indicate GFP::LGG-1 puncta. d Quantification of GFP::LGG-1 puncta in neuron from c. Values represent means ± s.e.m. (n = 3). P value (*P < 0.05) was determined by t-test. Scale bars, 20 μm (c)
