Fig. 1
From: Lack of androgen receptor SUMOylation results in male infertility due to epididymal dysfunction
ArKI targeting construct and expression of mutated AR. a Schematic representation of ArKI targeting construct. Two major SUMOylation sites, lysine in K381, K550 were mutated to arginines (K381R, K550R). X, generated XhoI site. b PCR used to detect WT and KI allele (lines 1−2 female; lines 3−5 male). c RT-qPCR analysis of Ar mRNA levels between ArKI and WT initial segment (IS) and caput (Cap) epididymidis and testis (n = 5 WT and 6 ArKI mice). Boxplot center lines indicate the median and the boxes extend to lower and upper quartiles with whiskers depicting maximum and minimum. d Western blotting of AR in ArKI and WT epididymis and testis. Immunoblotting of β-tubulin levels was used to control protein loading. e Immunofluorescent localization of AR in the IS/Cap epididymidis. Upper panels (red), AR; lower panels (white), DAPI. Scale bar 50 µm. AR androgen receptor, WT: wild type, KI: knockin, DAPI: 4′.6-diamidino-2-phenylindole
