Fig. 7

NK cells specifically eliminate Stat1^−/− T cells undergoing spontaneous proliferation. Rag1^−/− mice were injected with equal numbers (3–4 × 10⁶) of WT or Stat1^−/− CTV labeled unfractionated CD4⁺ T cells and analyzed after 1 week. a Schematic of experiment, as well as representative flow cytometry plots of T cells in the spleen + lymph nodes (gated on live CD45⁺ CD3ε⁺ CD4⁺ cells, Supplementary Fig. 4d) followed by their mean frequencies ± SEM. b, d Similar to a, but with 400μg anti-NK1.1 antibody or Isotype Control injected 1 day prior to T-cell transfer. b CTV profiles of the T cells are shown as well as the mean frequencies ± SEM of the SP population. c Representative images of FLICA staining from the T cell SP, HP and non-proliferating populations in a, as well as their mean frequencies ± SEM. d FLICA staining in the SP population from b shown with mean frequencies ± SEM shown. Pooled from three to four independent experiments, with each point representing an individual mouse. *p < 0.05, **p < 0.01, ***p < 0.001, ***p < 0.0001 by two-tailed Mann–Whitney test