Fig. 2
From: Sphingolipid-dependent Dscam sorting regulates axon segregation
Neuron class-specific role of sphingolipids in MB development. a Schematic showing the three classes of MB neurons. Green: γ, Red: α′/β′ and Blue: α/β. b Anterior View (Ventral (V) > Dorsal (D)) and Dorsal view (Anterior (A) > Posterior (P)) of the three differently labeled classes of MB neurons. γ: mCD8::GFP, α′/β′: mCherry, α/β: anti-FasII. Genotype: R26E01-LexA/LexAop myr::cherry; R16A06-Gal4/UAS-mCD8::GFP. R26E01-lexA (α′/β′ neurons), R16A06-Gal4 (γ neurons). c–h Morphology of MB in lacek05/ + (c, d, e) and lace2/lacek05 (f, g, h) class specifically labeled with mCD8::GFP for γ (c, f), α′/β′ (d, g), and α/β (e, h) neurons. α′/β′ and α/β neurons show a loss of the vertical axonal lobe in SPT mutants. n(c) = 26, n(d) = 26, n(e) = 38, n(f) = 42, n(g) = 36, n(h) = 36. i–s Schematic showing the development of different MB neuron classes (i). MB morphology in lacek05/ + (j–n) and lace2/lacek05 (o–s) at indicated developmental stages. Initially, γ neurons develop normally in SPT mutants ((j, o) Green). The axonal morphology defects first appear in α′/β′ neurons ((k, p) and (l, q) Green: α′/β′, Red: γ) followed by α/β neurons ((m, r) Green: α′/β′, Strong Red (FasII): α/β). MB are labeled with mCD8::GFP expressed using different Gal4s: 201Y- ((j, o) γ neurons), R30F11- ((k–m and p–r) α′/β′ and early born α/β) and OK107-Gal4 ((n, s) all classes of MB). n(j) = 38, n(k) = 12, n(l) = 6, n(m) = 16, n(n) = 48, n(o) = 30, n(p) = 26, n(q) = 8, n(r) = 10, n(s) = 52. c–s Green: GFP, Red: FasII, Blue: N-Cad. Scale Bar: 25 μm
