Fig. 7
From: Sphingolipid-dependent Dscam sorting regulates axon segregation
Dscam[TM1] interferes with Dscam[TM2] function in SPT mutant MB neurons. a Percentage of defective MB lobes in different indicated genetic backgrounds. In SPT mutants, knocking-down of Dscam[TM1] and expressing Vap33 using Dscam-Gal4 reduces axonal targeting defects while overexpression of Dscam[TM1] enhances it. Numbers on the bars represent number of MB analyzed. b, c Distribution of Dscam[TM2]::GFP at 20–25 h APF in the MB of lace2/ + (b) and lace2/lacek05 (c) expressed using 201Y-Gal4. While images b, c represent the axonal half of the MB, the corresponding somato-dendritic area is represented in right upper insets. The white dotted rectangle represents the Dscam[TM2]::GFP distribution in the axons, zoomed in and shown in the right lower inset. Green: Dscam[TM2]::GFP, Red: FasII, Blue: N-Cad. Arrowheads indicate somatic aggregates of Dscam. n(b) = 14, n(c) = 12. d–f MB expressing Dscam[TM1]::RFP (d), Dscam[TM2]::GFP (e) and Dscam[TM1]::RFP + Dscam[TM2]::GFP (f) using a α/β neuron-specific Gal4 (R65G04-Gal4). Insets show single channel fluorescence of Dscam[TM1] (d) /Dscam[TM2] (e) /Dscam [TM2] (f). n(d) = 26, n(e) = 26, n(f) = 28. g–j On expressing Dscam[TM1]::RFP (Red) with Dscam[TM2]::GFP (green) in lacek05/ + (g, i) and lace2/lacek05 (h, j) background with α/β specific Gal4 (R65G04-Gal4), Dscam[TM2] fails to localize to axons. n(g, i) = 28, n(h, j) = 30. d–j Green: Dscam[TM2]::GFP, Red: Dscam[TM1]::RFP, Blue: FasII, Arrowheads indicate somatic aggregates. Scale Bar: 25 μm
