Fig. 2
EDS1R493A delays TNL transcriptional reprogramming. a Four-week-old plants were infiltrated with Pst AvrRps4 (OD600–0.005) and free SA was quantified at 0, 8 and 24 hpi. Bars represent means ± SE of four biological replicates. Differences between genotypes were analysed using t-test (Bonferroni corrected, p < 0.05). Similar results were obtained in two independent experiments. b Four-week-old plants were infiltrated with 10 mM MgCl2 (mock) or Pst AvrRps4 (OD600–0.005), and leaf samples collected at 24 hpi. PR1 transcripts were measured using qRT-PCR and normalised to GapDH. Bars represent means ± SE of three biological replicates. Differences between genotypes were analysed using t-test (Bonferroni corrected, p < 0.05). c A 2-D scatter plot comparing R493A#1 vs. eds1-2 and R493A#1 vs. cEDS1 at 8 and 24 hpi with Pst AvrRps4. Plots depict differentially expressed genes (DEG) between R493A vs. eds1-2 (pink dots) and R493A vs. cEDS1 (blue dots). DEG represented were filtered with a |log2 FC| ≥ 1, FDR ≤ 0.05
