Fig. 4
Delayed immune signalling in R493A mutant plants is independent of COR. a Four-week-old plants were infiltrated with 10 mM MgCl2 (mock), Pst AvrRps4 or Pst ∆Cor AvrRps4 and free SA was quantified at 8 hpi. Bars represent means ± SE of three biological replicates. Difference between genotypes were analysed using t-test (Bonferroni corrected, p < 0.05). Similar results were observed in three independent experiments. b A multi-dimension scaling (MDS) plot of differentially expressed genes showing R493A transcriptional changes at 8 (open symbols) and 24 hpi (closed symbols). Encircled samples of eds1-2 (pink) and R493A (purple) highlight transcriptional trends with and without bacterial COR in Pst AvrRps4-triggered immunity. c A heatmap depicting DEG at 8 and 24 hpi normalised to Col (p < 0.05) after hierarchical clustering. Samples were harvested at 8 and 24 hpi with Pst AvrRps4 (Avr) and Pst ∆Cor AvrRps4 (∆Cor). Cluster #17 contains genes that are upregulated at 8 hpi with Pst ∆Cor AvrRps4 but not Pst AvrRps4 in R493A only. Expansion (right) highlights a subset of NLR and WRKY genes in cluster #17 involved in immunity whose expression is antagonised by COR in R493A (see Supplementary Table 3). d Circos plot showing overlap of 383 genes in cluster #17 with genes regulated by JA and Benzothiadiazole (BTH, an analogue of SA) from other studies (Hickmann et al.,2017; Yang et al.,2017). Genes differentially expressed in cluster #17 and other datasets are marked by connecting lines. Genes repressed by JA and induced by BTH (red and green lines converging to yellow, 113); genes repressed by JA and not expressed by BTH (green lines, 19); genes induced by BTH and not expressed in JA dataset (red lines, 169)
