Fig. 5
a positive charge at EDS1R493 is essential for TNL immunity. RPP4 resistance phenotypes of 2-week-old control and Arabidopsis transgenic lines expressing cEDS1 and R493 mutants, as indicated. Hpa EMWA1 infected leaves were stained with trypan blue at 5 dpi. Scale bar represents 100 μm. Each image is representative of > 18 leaves from two independent experiments. HR, hypersensitive response; fh, pathogen free hyphae. b Four-week-old Arabidopsis plants of the indicated genotypes were infiltrated with Pst AvrRps4 and Pst ∆Cor AvrRps4 (OD600–0.0005). Bacterial titres were determined at 3 dpi. Bars represent means of three biological replicates ± SE. Differences between genotypes were analysed using ANOVA (Tukey’s HSD, p < 0.05). Similar results were obtained in three independent experiments. c Four-week-old plants were infiltrated with Pst AvrRps4 or Pst ∆Cor AvrRps4 and free SA was quantified at 8 and 24 hpi. Bars represent means ± SE of three biological replicates. Differences between genotypes within treatments were analysed using Student’s t-test (Bonferroni corrected, ***p < 0.05) relative to Col. Similar results were obtained in two independent experiments
