Fig. 1
FTL9 is specifically induced under SDs in B. distachyon. a qRT-PCR analysis of six FT orthologous gene expressions at ZT4 under SDs and LDs. UBC18 was used as an internal control for normalization of qRT-PCR results. Plant newly expanded leaves were harvested for RNA isolation and gene expression analysis. Error bars indicate s.d. b Time course analysis of FTL9 expression at ZT4 when three-week-old SD plants were shifted to LDs for 1 day (S1L), 3 days (S3L), 7 days (S7L), and 14 days (S14L), respectively. S3w and S5w indicate that B. distachyon were grown under consistent SDs for 3 and 5 weeks. L3w indicates that B. distachyon were grown under LDs for 3 weeks as a control. UBC18 was used as an internal control for normalization of qRT-PCR results. Error bars represent s.d. c Time course analysis of FTL9 expression at ZT4 when three-week-old LD plants were shifted to SDs for 1 day (L1S), 3 days (L3S), 7 days (L7S) and 14 days (L14S), respectively. L3w and L5w indicate that B. distachyon were grown under consistent LDs for 3 and 5 weeks. S3w indicates that B. distachyon were grown under SDs for 3 weeks as a control. Error bars represent s.d. d qRT-PCR analysis of FTL9 expression under LDs in the wild-type Bd21-3 and phyc-1 homozyous mutants. UBC18 was used as an internal control for normalization of qRT-PCR results. Error bar means s.d. e qRT-PCR analysis of FTL9 expression under SDs in the wild-type Bd21-3 and phyc-1 homozyous mutants. Error bar means s.d.
