Fig. 3
FTL9-FAC has a weak flowering inductivity in B. distachyon. a The flowering inductive efficiency of FTL9-FAC is lower than that of FT1-FAC. Error bars indicate s.d. from different injected leaves. b FAC activity by FTL9 can be enhanced when the FTL9 PEBP domain is changed to that of FT1. PEBP domain of FTL9 is artificially swapped to that of FT1 and introduced to A.tumefaciens with FD1 and 3 kb VRN1 promoter-mediated LUC constructs, and co-infiltrated into N. benthamiana leaves. Error bars indicate s.d. c FAC activity by FT1 can be compromised when the FT1 PEBP domain is changed to that of FTL9. PEBP domain of FT1 is artificially swapped to that of FTL9 and introduced to A.tumefaciens with FD1 and 3 kb VRN1 promoter-mediated LUC constructs, and co-infiltrated into N. benthamiana leaves. Error bars indicate s.d. d Lysine at residue 128 of FTL9 is not responsible for the specific flowering ability of FTL9 in B. distachyon. Error bars indicate SD. (Student’s t test, *P < 0.05, **P < 0.01, ***P < 0.001) Left: Different combinations of effectors and the reporter transiently introduced in N. benthamiana leaves. Middle: Representative photograph of firefly luciferase fluorescence signals when the indicated reporter and effectors were introduced in N. benthamiana leaves. Right: Relative reporter activity (LUC/REN) in N. benthamiana leaves expressing the indicated reporters and effectors
