Fig. 4
Over-expression of FTL9 results in a flowering delay under LDs in B. distachyon. a Representative phenotypes of three FTL9 over-expressing lines under LDs. White arrows point to spikes. Scale bar, 5 cm. b Flowering time of wild-type Bd21 and the FTL9-OE plants under LDs. At least 10 plants for each line were scored. (Student’s t test, **P < 0.01, *P < 0.05). c qPCR analysis of flowering downstream gene VRN1 expression in four-week-old wild-type and the FTL9-OE plants under LDs. Error bars indicate s.d. d FTL9 attenuates the binding ability of FT1-FAC to VRN1 cis-elements. Left: Different combinations of effectors and the reporter transiently introduced in N. benthamiana leaves. Middle: Representative photograph of firefly luciferase fluorescence signals when the indicated reporter and effectors were introduced in N. benthamiana leaves. Right: Relative reporter activity (LUC/REN) in N. benthamiana leaves expressing the indicated reporters and effectors. Error bars indicate s.d. (Student’s t test, ***P < 0.001)
