Fig. 3
From: Allosteric enhancement of ORP1-mediated cholesterol transport by PI(4,5)P2/PI(3,4)P2
PI(3,4)P2/PI(4,5)P2 stimulates cholesterol transport by ORP1L in vitro. a SDS-PAGE of full-length ORP1L. b Lipid-binding analyses for ORP1L and its PH Domain. c, d DHE transport assays for ORP1-ORD (c) and ORP1L (d) with PIP embedded in the donor liposomes. Shown at the bottom are the initial velocities. e PI(4,5)P2 (left) and PI4P (right) transport assays for ORP1L. (For all bar graphs, data are shown as mean ± s.e.m. (error bar), n = 3)
