Fig. 4
From: Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader
Performance of RAPTER in undiluted clinical serum samples. a CV scan of the LH aptamer sensor in PBS and then in undiluted hypothalamic amenorrhoea serum. b SWV of the LH aptamer sensor in PBS and then in undiluted hypothalamic amenorrhoea serum. c Regeneration under an undiluted serum condition. The dashed line represents the regenerated peak obtained after using the automatic platform washing step. d Concentration–response curve using SWV methods. The % of the signal change indicates that the change is a signal off model. The KD is estimated to be 358 nM, and the dynamic range is from 5 to 500 nM. Means ± s.d. from three independent experiments are shown (n = 3). e Pilot study on measuring LH concentration. Six samples with a known wide variation in the range of LH concentration measured by the aptamer and clinical gold standard immunochemical assay. Bland–Altman plot and linear regression plot of the LH concentration obtained by the two methods, R2 = 0.93. Source data are provided as a Source Data file
