Fig. 4
From: Design strategy for serine hydroxymethyltransferase probes based on retro-aldol-type reaction
Fluorescent probe targeting hSHMT. a Schematic illustration of hSHMT fluorescent probe 1. b Fluorescence spectral change of probe 1 (4.6 μM) during the hSHMT1-catalyzed reaction from 0 to 60 min. Excitation at 390 nm. Assay conditions: 5 units/mL hSHMT1, 50 mM HEPES buffer (pH 7.5), 100 mM NaCl, 0.5 mM EDTA, 1 mM dithiothreitol (DTT), with or without inhibitor (±)-SHIN1 10 µM, 0.6% DMSO, 37 °C. The inset shows the fluorescence change of probe 1 (5 μM). c Conversion rate analysis of dl-erythro, dl-threo, or L-erythro probes (4.6 µM) by time-dependent fluorescence analysis at 530 nm (excitation at 390 nm). I0 at 530 nm is the fluorescence intensity under the condition without hSHMT1. Source data are provided as a Source Data file
